Detailed Abstract
[Poster Presentation 5 - Biliary & Pancreas (Biliary Disease/Surgery)]
[PP 5-4] ARTIFICIAL INTELLIGENCE-POWERED SPATIAL ANALYSIS OF TUMOR-INFILTRATING LYMPHOCYTES PREDICTS PROGNOSIS IN RESECTED GALLBLADDER CANCER
So Jeong YOON 1, Young Hoon CHOI 2, Hyemin KIM 2, Se-Hoon LEE 2, Kwang Hyuck LEE 2, Jong Kyun LEE 2, Kyu Taek LEE 2, Sang Hyun SHIN 1, In Woong HAN 1, Jin Seok HEO 1, Kee-Taek JANG 3, Joo Kyung PARK 2, Hongbeom KIM 1
1 Division of Hepatobiliary-Pancreatic Surgery, Department of Surgery, Samsung Medical Center, Sungkyunkwan University, Korea, 2 Department of Medicine, Samsung Medical Center, Sungkyunkwan University, Korea, 3 Department of Pathology And Translational Genomics, Samsung Medical Center, Sungkyunkwan University, Korea
Background : Tumor infiltrating lymphocytes (TIL) play an essential role in various cancer types. However, their prognostic significance in resected gallbladder cancer remains largely unknown.
Methods : This study included 225 patients who underwent curative resection for gallbladder cancer. H&E-stained whole slide images were analyzed using the Lunit SCOPE IO, an artificial intelligence (AI)-powered spatial TIL analyzer. Based on the density of intratumoral TILs in the cancer epithelium and stromal TILs in the cancer stroma, three immune phenotypes (IP) were defined: immune-inflamed phenotype (IIP), immune-excluded phenotype (IEP), and immune-desert phenotype (IDP). Survival outcomes were analyzed, with subgroup analysis conducted for early-stage (stage 0-II) and advanced-stage (stage III-IV) disease.
Results : Of the 225 patients, 58 (25.8%) had IIP, 151 (67.1%) had IEP, and 16 (7.1%) had IDP. During a median follow-up of 71 months, 82 deaths (36.4%) were recorded. Both IIP and IEP were significantly associated with longer disease-free survival (DFS) and overall survival (OS) compared to IDP.
Conclusions : Immune phenotypes identified through AI-powered spatial TIL analysis were associated with DFS and OS in resected gallbladder cancer, particularly in late-stage disease. These findings highlight the prognostic potential of immune phenotyping, especially in advanced diseases.
Methods : This study included 225 patients who underwent curative resection for gallbladder cancer. H&E-stained whole slide images were analyzed using the Lunit SCOPE IO, an artificial intelligence (AI)-powered spatial TIL analyzer. Based on the density of intratumoral TILs in the cancer epithelium and stromal TILs in the cancer stroma, three immune phenotypes (IP) were defined: immune-inflamed phenotype (IIP), immune-excluded phenotype (IEP), and immune-desert phenotype (IDP). Survival outcomes were analyzed, with subgroup analysis conducted for early-stage (stage 0-II) and advanced-stage (stage III-IV) disease.
Results : Of the 225 patients, 58 (25.8%) had IIP, 151 (67.1%) had IEP, and 16 (7.1%) had IDP. During a median follow-up of 71 months, 82 deaths (36.4%) were recorded. Both IIP and IEP were significantly associated with longer disease-free survival (DFS) and overall survival (OS) compared to IDP.
Conclusions : Immune phenotypes identified through AI-powered spatial TIL analysis were associated with DFS and OS in resected gallbladder cancer, particularly in late-stage disease. These findings highlight the prognostic potential of immune phenotyping, especially in advanced diseases.
SESSION
Poster Presentation 5
Exhibition Hall 3/27/2025 2:50 PM - 3:30 PM